Membrane Proteins

  1. Brian L. Cook, Karin E. Ernberg, Hyeyoun Chung, Shuguang Zhang. Study of a synthetic human olfactory receptor 17-4: Expression and purification from an inducible mammalian cell line. PLoS ONE, 2008, 3 (8), e2920.  [local pdf]

  2. Liselotte Kaiser, Johanna Graveland-Bikker, Dirk Steuerwald, Melanie Vanberghem, Kara Herlihy, Shuguang Zhang. Efficient cell-free production of olfactory receptors: Detergent optimization, structure, and ligand binding analyses. Proc. Natl. Acad. Sci. USA, 2008,  105 (41), 15726–15731. [local pdf]

  3. Hidehito Takayama, Prashen Chelikani, Philip J. Reeves, Shuguang Zhang, H. Gobind Khorana. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE, 2008, 3 (6), e2314. [local pdf]

  4. Brian L. Cook, Dirk Steuerwald, Liselotte Kaiser, Johanna Graveland-Bikker, Melanie Vanberghem, Allison P. Berke, Kara Herlihy, Horst Pick, Horst Vogel, Shuguang Zhang. Large-scale production and study of a synthetic G protein-coupled receptor: Human olfactory receptor 17-4. Proc. Natl. Acad. Sci. USA 2009 Vol 106, 11925-11930 . [local pdf]

  5. Hui Ren, Daoyong Yu, Baosheng Ge, Brian Cook, Zhinan Xu, Shuguang Zhang. High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1. PLoS ONE, 2009, 4 (2), e4509. [local pdf]

  6. Hanna M. Eriksson, Karina Persson, Shuguang Zhang, Ake Wieslander. High-yield expression and purification of a monotopic membrane glycosyltransferase. Protein Expression and Purification, 2009, 66, 143–148. [local pdf]

  7. Kwong-Joo Leck, Shuguang Zhang, Charlotte A. E. Hauser. Study of bioengineered zebra fish olfactory receptor 131-2: Receptor purification and secondary structure analysis. PLoS ONE, 2010, 5 (11), e1527. [local pdf]

  8. Karolina Corin, Philipp Baaske, Deepali B. Ravel, Junyao Song, Emily Brown, Xiaoqiang Wang, Sandra Geissler, Christoph J. Wienken, Moran Jerabek-Willemsen, Stefan Duhr, Dieter Braun, Shuguang Zhang. A robust and rapid method of producing soluble, stable, and functional G-Protein Coupled Receptors. PLoS ONE, 2011, 6 (10), e23036. [local pdf]

  9. Xiaoqiang Wang, Shuguang Zhang. Production of a bioengineered G-protein coupled receptor of human formyl peptide receptor 3. PLoS ONE, 2011, 6 (8), e23076. [local pdf]

  10. Xiaoqiang Wang, Karolina Corin, Philipp Baaske, Christoph J. Wienken, Moran Jerabek-Willemsen, Stefan Duhr, Dieter Braun, and Shuguang Zhang. Peptide surfactants for cell-free production of functional G protein-coupled receptors. Proc. Natl. Acad. Sci. USA, 2011, 108 (22), 9049–9054. [local pdf]

  11. Gang Yang, Gosta Winberg, Hui Ren, Shuguang Zhang. Expression, purification and functional analysis of an odorant binding protein AaegOBP22 from Aedes aegypti. Protein Expression and Purification, 75 (2011) 165–171. [local pdf]

  12. Karolina Corin, Philipp Baaske, Deepali B. Ravel, Junyao Song, Emily Brown, Xiaoqiang Wang, Christoph J. Wienken, Moran Jerabek-Willemsen, Stefan Duhr, Yuan Luo, Dieter Braun, Shuguang Zhang. Designer lipid-like peptides: A class of detergents for studying functional olfactory receptors using commercial cell-free systems. PLoS ONE, 2011, 6 (11), e25067. [local pdf]

  13. Karolina Corin, Philipp Baaske, Sandra Geissler, Christoph J. Wienken, Stefan Duhr, Dieter Braun, Shuguang Zhang. Structure and function analyses of the purified GPCR human vomeronasal type 1 receptor 1. Nature Publishing Group: Scientific Reports, 2011, 1, e172. [local pdf]

  14. Xiaoqiang Wang, Karolina Corin, Cyrus Rich, Shuguang Zhang. Study of two G-protein coupled receptor variants of human trace amine-associated receptor 5. Nature Publishing Group: Scientific Reports 2011 1, e102. [local pdf]

  15. Karolina Corin, Horst Pick, Philipp Baaske, Brian L. Cook, Stefan Duhr, Christoph J. Wienken, Dieter Braun, Horst Vogel, Shuguang Zhang. Insertion of T4-lysozyme (T4L) can be a useful tool for studying olfactory-related GPCRs. Molecular BioSystems, 2012, 8, 1750–1759. [local pdf]

  16. Karolina Corin, Xiaoqiang Wang, Shuguang Zhang. Production of olfactory receptors using commercial E. coli cell-free systems. In: Tai Hyun Park, Ed., Bioelectronic Nose: Integration of Biotechnology and Nanotechnology.  Springer, 2014, Chapter 7, pp 115-126. [local pdf]

  17. Lotta Tollstoy Tegler, Karolina Corin, Julia Hillger, Brooke Wassie, Yanmei Yu, Shuguang Zhang. Cell-free expression, purification, and ligand-binding analysis of Drosophila melanogaster olfactory receptors DmOR67a, DmOR85b and DmORCO. Nature Publishing Group: Scientific Reports, 2015 5, 7867; DOI:10.1038/srep07867. [local pdf]